Neuropeptide Y in Spotted Scat (Scatophagus Argus), Characterization and Functional Analysis towards Feed Intake Regulation

Neuropeptide Y (Npy) is an intricate neuropeptide regulating numerous physiological processes. It a highly conserved peptide known to improve feed intake in many vertebrates, including fishes. To enlighten the mechanism of Npy spotted scat control, we cloned and identified cDNA sequence. We further examined its expression some tissues explored effects at different time frames (hours days). Here, discovered that comprised 300 bp open reading frame (ORF) 99 amino acid was be expressed all examined. Using situ hybridization examination, proved npy has wide brain scat. Furthermore, hypothalamus significantly increased one hour after feeding (p < 0.05). Further, it revealed fish were fasted for up 5 days refeeding from 8th 10th day. This suggests orexigenic peptide, hence, increases food growth Additionally, results vitro vivo experiments locally interacts with other appetite-regulating peptides hypothalamus. research aimed set fundamental study developing regulation, improving reproduction, which significant aquaculture industry